Players can collect various items from the Mario universe. Press the A button when next to the item to trigger its special action.
- brick block
- cannon - shoots a Bullet Bill
- coin - makes the Super Mario Bros. "coin" sound effect
- fire bar - continuously plays the SMB castle theme*
- fire flower - plays fireball sound effect
- flagpole - plays "down the flagpole" sound effect from SMB
- green pipe - plays the "entering a pipe" sound effect; Piranha Plant moves up and down inside pipe
- koopa shell - plays the shell-kick sound effect
- 1-Up mushroom - plays 1-Up theme as "1UP" text floats above it
- ? block - plays "vine growing" sound effect
- starman - plays invincibility theme*
- super mushroom - plays power-up sound effect
- mushroom mural - wallpaper based on SMB World 2-1 background
- block floor - carpet with SMB's block-floor pattern
*The fire bar and starman play music without any player input.
In the U.S., Toys "R" Us stores gave away six of the Mario items from July 23, 2006 to August 1, 2006 using special Nintendo DS kiosk machines. These items were received from bottle mail that featured Mario's face in the background as you read the letter.
You can dress up your character with Mario-themed clothing and accessories, some of which are also in the GameCube Animal Crossing.
- big bro's shirt - a red shirt with Mario's "M" logo
- li'l bro's shirt - a green shirt with Luigi's "L" logo
- big bro's hat - Mario's hat
- li'l bro's hat - Luigi's hat
- big bro mustache - a Mario nose-and-mustache combo
- Toad parasol - a red umbrella with white dots (Toad's hat colors reversed)
- Toad shirt - a red shirt with white dots (known as "toad print" in the GameCube version)
One of the stationery designs is called "SMB3 paper" and resembles the Super Mario Bros. 3 title screen.
The "arcade machine" shows Luigi and Mario facing each other in a fighting game. "Nintendo" is written in white letters on both sides of the cabinet.
The "pinball machine" has the artwork from the Japanese Super Mario Bros. packaging on its backglass and a picture of Mario on its lower playfield. The pictures show up much clearer in Animal Crossing: City Folk.
One rock per day yields multiple bags of bells when struck with your shovel. For a limited time, each whack with your shovel will knock out a bag. After four bags come out, the classic Mario 1-Up tune will play for each subsequent bag that you knock out.