One of the "geeks" (whose name is Mario, incidentally) for his comedy routine talked about Super Mario Bros. and its catchy music, which he then sang.

This episode centers around the show's various characters using the Professor's "What If?" machine to show them various alternate realities. Fry wants to know what life would be like if it was more like a video game. The machine shows a world where Earth goes to war with the Planet Nintendu 64 (yes, Nintendu). At the start of the scenario, President Nixon is signing a peace treaty with Donkey Kong, who is the Nintendu 64ian ambassador to Earth. DK turns on Nixon, however, and Mario, who is part of the Earth U.N., expresses fear.

Mario: Mamma mia! The cruel meatball of war has-a rolled onto our laps, and-a ruined our white pants of peace.

Donkey Kong's scenes are too numerous to mention, but here's a nice exchange:

Fry: What do you monsters want?
Donkey Kong: One thing, and one thing only. Quarters! A million allowances worth of quarters! No slots or tokens!
Monsters: Fork 'em over. Fork 'em over.
Professor: Forget it you pixelated pirates! We need those quarters to do our laundry.
Lur: But, but, space invaders need to do laundry too. I mean, look at Donkey Kong here. Have you smelled his loin cloth lately?

When Earl's friends list great things about the United States to foreigner Pierre, Randy (Ethan Suplee) mentions Donkey Kong along with several other video games: "Pac-Man, Donkey Kong, Dig Dug, Mrs. Pac-Man, Frogger, Mrs. Frogger -- we don't have that one yet, but I'm sure the scientists are workin' on it."

During the Weekend Update skit, a news item about Christian Video Games ends with "Super Jesuit Bros", accompanied by a picture of Mario as a Jesuit. The picture used was converted from Super Mario Sunshine art.

Wario, holding a shotgun, marches to battle along with other evil characters.
While good characters prepare for war, Mario grabs a sword from the armory.
As the two sides are about to clash on the battlefield, Wario (now holding a spear) and Bowser are shown running towards the screen.

Wario lookalike with "V" on his hat is among the crowd of evil Imaginationland people watching Strawberry Shortcake get attacked.

Mario lookalike with "E" on his hat (lowercase "m" turned on its side) is seen among the Imaginationland inhabitants.

A screen shot from Super Mario Bros. 2 and another from Super Mario Bros. are displayed on two arcade machines inside Blanket's house. Only visible for a few seconds, they are part of a group of four arcade games with generic-looking cabinets and obviously fake titles. The machine with SMB2 has the title "______ ___ Blood." SMB is titled "Farmer's Market" and has a steering wheel. (Another NES game, Zelda II: The Adventure of Link, is two machines down from SMB. We're not sure what game's screen shot is on the "Space Brain Damage" machine between SMB and Zelda II.)

While Letterman and a John Madden impersonator (Frank Caliendo) discuss the new Madden 07 video game, "Madden" reminisces about Donkey Kong and Super Mario Bros.

At the "Try-N-Save," Bart encounters a pair of his school mates, who brag that they are currently shoplifting. After they walk away, Bart notices that the video game display case has been left open. As Bart looks at a game called "Bonestorm," he ponders stealing it. One after another, several video game characters appear as his conscience:

Luigi: Go ahead-a, Bart, take-a the Bonestorm.
Mario: The store, she's so rich. She'll-a never notice.
Donkey Kong: Duh, it's the company's fault for making you want it so much.
Lee Carvallo: Don't do it, son. How's that game going to help your putting?
Sonic: Just take it! Take it, take it, take it, take it, take it! TAKE IT!

(Voice of Mario: Dan Castellaneta. Voice of Luigi: Hank Azaria. Voice of DK: Harry Shearer.)

At the Noiseland Arcade, Donkey Kong sits under a sign that reads "Friday Meet Donkey Kong in Person." While holding a cigarette, DK picks a bug off his leg and eats it. Then, the Manager walks by.

Manager: Sorry Donkey Kong, you're just not a draw anymore.
He walks away. Donkey Kong throws a barrel at him as a generic video game sound effect plays. The Manager is knocked to the ground.
Manager: Hey, he's still got it!

A bus of "Italians Touring America" drives by Homer's house. The tour guide tells the passengers to, "Look at the American computer monkey! Dance-a monkey, dance!" Homer makes some threatening monkey gestures at them. Mario, who's a passenger on the bus, runs out and says, "Busta, busta [??], take-a my picture with the internet marmalok. [!?]" Homer starts throwing his conveniently-placed trash cans at Mario and a full-scale Donkey Kong battle takes place, complete with sound effects, the hammer, and Mario's classic death animation.

During Springfield Elementary School's medieval festival, Principal Skinner has Groundskeeper Willie in a cage, explaining, "Since the Middle Ages had no compact discs or Super Mario men, people found entertainment abusing the village idiot."

Carmela Soprano (Edie Falco) punishes A.J. (Robert Iler) for getting suspended from school: "You are not to play Mario Kart or go skateboarding for three weeks."