It mostly depends on how you define "mute."
Mario is the one that fits the concept the most loosely (I used a loose definition of "mute" for the whole comic, to be honest); he does talk, but it's sparse enough that I felt like I could get away with inserting him into the joke.
Kirby and Link don't ever speak which fits the aforementioned loose definition of "mute."
Crash almost never even opens his mouth, except in the opening screen for the first game, in which he screams for less than one second (he says "whuh-oh!" in the intro movie, but most people I've talked to don't even know that movie exists).
Bluu hasn't talked yet, but I'm thinking about upgrading him to a spoken role in the sequel I'm working on, rather like Naughty Dog did with Jak. Unlike Jak's script, I can guarantee that if I do decide to do this, his dialogue will be strictly PG.